![]() It accounts for the immersion, the cover-slip and the sample layers. This model describes the scalar-based diffraction that occurs in the microscope. This PSFGenerator is based on a previous version including 7 PSF models and running only as a ImageJ plugin. In addition, we expect you to include adequate citations and acknowledgments whenever you present or publish results that are based on it. You are free to use this software for research purposes, but you should not redistribute it without our consent. > psf = pute( 'config.txt') % Compute a PSF using the parameters of config.txt (without GUI)Īll the parameters are stored in a human-readable text file config.txt. > psf = PSFGenerator.get % Compute a PSF from the GUI parameters > PSFGenerator.gui % with Graphical User Interface > javaaddpath /Applications/MATLAB_R2013a.app/java/PSFGenerator.jar To run with the GUI enter on the Matlab console: Download PSFGenerator.jar and save the file in the java folder of Matlab. PSFGenerator is a set of Java classes callable from Matlab. Java -cp PSFGenerator.jar PSFGenerator config_filename.txt Download PSFGenerator.jar and from the terminal type: java -cp PSFGenerator.jar PSFGenerator The plugin consist in one single JAR file place it into the "plugins" folder of Fiji. PSFGenerator is a plugin for Fiji, a distribution of ImageJ and ImageJ2 running on Linux, Windows, and Mac OSX.ĭownload PSF_Generator.jar. The plugin consist in one single JAR file place it into the "plugins" folder of ImageJ. PSFGenerator is a plugin for ImageJ, a general purpose image-processing and image-analysis package running on Linux, Windows, and Mac OSX.ĭownload PSF_Generator.jar. ![]() Go to Online plugin of Icy and installed PSFGenerator. The installation of the plugin is performed through the centralized distribution service of Icy. PSFGenerator is a plugin for Icy, an open community platform for bioimage informatics running on Linux, Windows, and Mac OSX. Download ObjectĮxample of Gibson and Lanni PSF Holden, " Super-Resolution Fight Club: Assessment of 2D and 3D Single-Molecule Localization Microscopy Software," Nature Methods-Techniques for Life Scientists and Chemists, vol. Unser, " Quantitative Evaluation of Software Packages for Single-Molecule Localization Microscopy," Nature Methods-, vol. Unser, " DeconvolutionLab2: An Open-Source Software for Deconvolution Microscopy," Methods-, vol. A User Point of View, Part I and Part II, G.I.T. Sage, Comparison of Deconvolution Software in 3D Microscopy. Unser, 3-D PSF Fitting for Fluorescence Microscopy: Implementation and Localization Application, Journal of Microscopy, vol. Potential applications are 3D deconvolution, 3D particle localization and tracking, and extended depth of field The computational tasks and on a numerical method that adapts to the oscillatory nature of the required integrands. Our Java implementation achieves fast execution times, as it is based on multi-threading The program requires only few parameters which are readily-available for microscopy practitioners. PSF Generator is provided for several environments: as ImageJ/ Fiji plugin, as an Icy plugin, and as a Java standalone application. Optical Transfer Function generated in the Fourier domain: Koehler simulation, defocus simulation. "Gaussian", "Lorentz", "Cardinale-Sine", "Cosine", "Circular-Pupil", "Astigmatism", "Oriented-Gaussian", "Double-Helix". ![]() Scalar-based diffraction model with 3 layers Gibson & Lanni,Īnd vectorial-based model Richards & Wolf,Īnd Variable Refractive Index Gibson & Lanni model.ĭefocussing a 2D lateral function with 1D axial function: the available lateral functions are: The current version has more than fifteen different models:ģD diffractive models: scalar-based diffraction model Born & Wolf, PSF Generator is a software package that allows one to generate and visualize various 3D models of a microscope PSF. ![]()
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